A known amount of amphiphilic molecules is disolved in a spreading solvent. A few microliters of the solution are dripped onto the water with a micro syringe. The solvent evaporates and a welldefined amout of amphiphiles remains on the surface.
A simple parameter of the system is the
Phase Transition of the system, i.e. the chain ordering transition of lipids, appear in the isotherm as regions of constant pressure - a first order phase transion with a coexistence of two phases - or as kink in the isotherm - a second order phase transition. The recording of isotherms and the determination of phase transitions is the basic effort in order to characterise the system. Then other techniques can be applied
Aside form the area per molecule other external pareameters can be varied on a langmuir trough: Temperature, content of salt, polymers or proteins in the subphase, light organic molecules can be added on the airside, ...
Surface experiments are threatenend by even tiny concentrations of impurities. Hence experiments on a langmuir trough are mainly: Cleaning. Repeated filling with water of high purity, followed by sucking off, after application of tensid or propanol solutions and wiping with solvents takes most of the time. The measurenment itself on the other hand is mainly computer controlled and requires little attention.
«Amphiphilic molecules consist of water loving, i.e. polar or ionic, and a water repelling, nonpolar part. A simple example are fatty acids. In experiments on a langmuir trough the molecule must be bound to the surface, at least metastable. This requires little or no solubility in water, achieved by a sufficently large hydrophobic part.
« A solvent spreads, if the surface energy of the air/water interface is larger than the surface energies of the solvent/water and the solvent/air interfaces together. In this case the thin film formation is energtically favoured. The spreading of this film also distributes the molecules on the surface. A typical solvent is chloroform or mixtures of chloroform with small shares of polar molecules (methanole, ethanole). These additives increase the solubility of amphiphil molecules.